Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells with cell population frequency information
Cryopreserved ampule of Normal Human Dendritic Cells (NHDC) containing ≥2.5 million cells
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format).
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For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector® X Unit (20 µL format).
Do you need a quote right away? Add this product to your shopping cart, go to your cart and click on “Generate Quote”. Your quote will be available straight away to view, save online, and download in PDF format.
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector® X Unit (100 µL format).
Human CD8+/CD45RA+ naïve cytotoxic T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
Human CD4+/CD45RA+ Naïve T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
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Terms and ConditionsCD34+ cells BOGO
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)