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Primary Cell Optimization 4D-Nucleofector® X Kit
Catalog #: V4XP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
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Primary Cell Optimization 384-well Nucleofector® Kit
Catalog #: V5SP-9001
For high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector® System
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Stellate Cells, Human Cryopreserved, 0.2 Million Cells
Catalog #: HUCLS-200K
Low passage stellate cells dervied from human liver tissue containing 200,000 cells/vial
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Stellate Cells, Human Cryopreserved, 1 Million Cells
Catalog #: HUCLS-1M
Low passage stellate cells dervied from human liver tissue containing 1 million cells/vial
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Human Bone Marrow Stromal Cells, 5 Million
Catalog #: 2M-302
Cryopreserved ampule of Human Bone Marrow Stromal Cells containing ≥5 million cells
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Human Bone Marrow Mononuclear Cells MNCs, Cryopreserved, 25 Million Cells
Catalog #: 2M-125C
Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing ≥25 million cells
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Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million
Catalog #: 2M-101C
Bone Marrow CD34+ Cells, 1 Million, Cryopreserved, Positively Selected
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Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 2 Million
Catalog #: 2M-101D
Bone Marrow CD34+ Cells, ≥2 million cells, cryopreserved, positively selected
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P3 Primary Cell 4D-Nucleofector® X Kit L
Catalog #: V4XP-3024
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format).
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Human Subcutaneous Preadipocyte Cells, 1 Million
Catalog #: PT-5020
Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥1 million cells
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Instructions - Cryopreserved Kupffer CellsInstructions for Thawing and Plating of Human Cryopreserved Kupffer Cells
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NPCs Hepatic Non-Parenchymal CellsThese additional liver cell types provide a complete building set supporting complex cell culture modeling. Applications include developing more physiologically relevant in vitro models for complex liver diseases and toxicity testing of new drugs and chemicals.
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The Importance of Cell-subtype Selection in Creation of a 3D Liver Fibrosis ModelData sheet detailing 3D liver fibrosis model in collaboration with Visikol
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Inventory - Non-Parenchymal CellsListing of available non-parenchymal cells inventory; updated monthly March 2019 NPC Inventory
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Primary cells and other tools for in vitro ADME-ToxCD-LI019_ADME-Tox_Product_listing_sec.pdf
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Engineering Culture Platforms to Mimic Liver DiseasesLearn from Dr. Salman Khetani, University of Illinois at Chicago, how multiple liver cell-types including hepatocytes, Kupffer, stellate, and endothelial cells can be engineered together to model human liver disease phenotypes in culture. -
TechSheet – Dulbecco’s Phosphate Buffered Saline (DPBS)Product use of Dulbeccos Phosphate Buffered Saline (DPBS).
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A Novel In Vitro Liver Cell Culture Flow System Allowing Long-Term Metabolism and Hepatotoxicity Studies]Manuscript published in Applied In Vitro Toxicology journal describes improvement of CYP450 phenotype of primary cryopreserved hepatocytes using Quasi Vivo® System
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Endothelial Cells Concentration Guide
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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