For use in combination with TheraPEAK® Nucleofector® Solution Sets, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.
Cryopreserved ampule of Adult Human Dermal Microvascular Endothelial Cells (HMVEC-d Ad) containing ≥ 500,000 cells
EGM®-2 MV Microvascular Endothelial Cell Growth Medium-2 SingleQuots® Kit contains all supplements and growth factors required for Microvascular Endothelial Cells (basal media not included)
Basal media required for growth of endothelial cells, phenol red free, 500 mL (supplements and growth factors sold separately)
Culture system containing EBM®-Plus Basal Medium (CC-5036) and EGM®-Plus SingleQuots® supplements (CC-4542) required for growth of Endothelial cells. This media is optimized for use with human umbilical vein endothelial cells (CC-2935).
FGM®-2 Fibroblast SingleQuots® Kit
Supplements and growth factors required for culture of endothelial cells (basal media sold separately)
Cryopreserved ampule of Neonatal Normal Human Epidermal Keratinocytes (NHEK-Neo), Single Donor, containing ≥ 500,000 cells
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TechSheet - Cardiac Fibroblast Cell SystemsTechnical Use and Product Information for CC-2903
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TechSheet – Calcium SingleQuots™ (CC-4202)Technical information sheet for Clonetics™ Calcium SingleQuots™
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Tips For Optimizing Your CD8+ T Cell and Dendritic Cell Co-culturesA co-culture between matured dendritic cells (DCs) and CD8+ T cells isolated from Lonza cryopreserved human peripheral blood mononuclear cells (PBMCs; part nos. CC-2702 – CC-2705) and we present three major takeaways from this process that are critical for successful co-culture and end-point analysis for these two cell types
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TechSheet – Veronal BufferHow to use Veronal Buffer (12-624)
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TechSheet – Trypan Blue Stain 0.4%Product information on Trypan Blue (17-942)
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TechSheet – Trypsin/Trypsin-Versene® (EDTA)/Versene® (EDTA)Overview of different Trypsin solutions
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TechSheet - Rat Calvariae Osteoblast Cell SystemTechnical tips for culturing Rat Calvariae Osteoblasts
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ProSieve™ Unstained Protein Marker II - ProtocolInstructions for proper use of ProSieve™ Unstained Protein Markers
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Cell Counting and Determination of Viability Via HemocytometerTechnical Reference guide cell counting
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Endothelial Cells Concentration Guide