The kinetic chromogenic LAL method is based on the measurement of color at different intervals of time after the addition of the chromogenic substrate (containing the LAL reagent and chromogenic reagent) to the test sample. The solution is monitored over time for the appearance of the yellow color, which is measured using spectrophotometric methods. The amount of time required for the color change to occur is inversely proportional to the amount of endotoxin present. The concentration of unknown samples can then be calculated by interpolating into a standard curve. Some important advantages of this method are that it can be totally automated to increase testing efficiency, and that it allows for the measurement of many samples in a short period of time via easy data processing.
Our Kinetic-QCLTM Kinetic Chromogenic LAL Assay provides a quantitative, kinetic chromogenic BET method and is the most sensitive of the LAL assays, with a sensitivity range of 0.005 EU/ml - 50.0 EU/ml. The test sample is mixed with the chromogenic substrate in a 96-well plate. This is then placed in an incubating absorbance plate reader that measures absorbance at 405 nm.