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Cell Line Nucleofector™ Kit R

Catalog #: VCA-1001
For efficient transfection of cell lines, e.g. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the NucleofectorTM I/II/2b Device
 683.00 CHF
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Product Overview

The Cell Line NucleofectorTM Kit R is one of our five NucleofectorTM Kits suited for transfecting cell lines in the NucleofectorTM 2b Device. This kit has been determined to be optimal for transfecting following cell types:

B16-F0
BJ
COS-7
HeLa
HT-29
Hut-78
IMR-90
KG-1
L6
LNCaP
MDBK
NIH/3T3
NK-92
PANC-1
Sf9
WI-38 

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

 

Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines

Applications

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Content & Storage

Content

2.25 mL NucleofectorTM Solution R
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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