SeaKem® Gold Agarose
SeaKem® Gold Agarose
SeaKem® Gold Agarose, 125 g, ideal for separation of large DNA fragments using low concentration gels or PFGE.
Product Overview
SeaKem® Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology GradeTM Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem® Gold Agarose gels is significantly greater than in conventional agarose gels. Run times for PFGE can be decreased by as much as 50% depending upon buffer and agarose concentration. Due to its high gel strength, SeaKem® Gold Agarose forms easy-to-handle gels at low concentrations (as low as 0.5%), which allow for the separation of larger DNA fragments by conventional electrophoresis as well as a decrease in the time needed to separate DNA by PFGE.
Analytical Specifications
Gelling temperature (1.5%) 36°C ±1.5°C
Melting temperature (1.5%) ≥90°C
Gel strength (1.0%) ≥1,800 g/cm²
Gel strength (1.5%) ≥3,500 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
- All Lonza Agarose is quality tested to certify performance
- Highest gel strengh
- Low EEO
- Fast separations of megabase sized DNA fragments using PFGE
Applications
- PFGE
- Large Fragment Electrophoresis
Storage and Content
- SeaKem® Gold Agarose 125 g
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
-
SourceBook Section XIII - Protein Separation in Agarose Gels
1. Introduction 2. Buffers for Protein Separation in Agarose 3. Casting Agarose Gels for Protein Separation 4. Preparation and Loading of Protein Samples 5. Optimal Voltage and Electrophoretic Times 6. Detection of Proteins in Agarose Gels 7. Gel Drying and Preservation 8. Processing Agarose Gels Following Electrophoresis 9. Recovering Proteins from Agarose Gels 10. References -
SourceBook Appendix B - Agarose Physical Chemistry
1. Agarose Physical Chemistry 2. References -
SourceBook Appendix A - Electrophoretic Theory
1. Voltage, Current and Power; Interactive Effects on Gel Electrophoresis 2. Electrophoretic Parameters -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
SourceBook Section IX - Special Applications in Agarose Gels
1. Amplification of Plasmid cDNA Libraries with SeaPrep® Agarose 2. Preparing Agarose for use in Cell Culture Applications 3. References -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
SourceBook Section VI - Recovery of DNA from Agarose Gels
1. Tips for Increasing DNA Recovery Efficiency from Agarose Gels 2. b-Agarose Recovery of DNA from Agarose Gels 3. Electroelution of DNA from Agarose Gels 4. Phenol/Chloroform Extraction of DNA from Agarose Gels 5. "Modified Freeze/Squeeze" Extraction of DNA from Agarose Gels 6. Ethanol Precipitation of DNA Recovered from Agarose Gels 7. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
