Single-cell type organoids
Multiple-cell type organoids
Multiple-cell type organoids
Speroids and tumoroids
Viability staining and morphology of the islets in different culture conditions after the indicated time points detected by confocal laser scanning microscopy. (a, b) shows monolayer cultures, (c, d) RAFTTM Cultures, (e,f columns) suspension cultures. Between days 4 and 7, gradual morphological changes were noticed with the outspreading of fibroblast-like cells in the monolayer cultures (a, b columns),whereas islets inoculated directly within RAFTTM Gels preserved their globular shape (c,d columns). Between day 10 and 18 the continued outgrowth of fibroblast-like cells resulted in the loss of islet integrity in monolayer cultures (a, b)whereas RAFTTM Cultures maintained spheroid structures (c, d). Data courtesy of Szebeni et al Cytotechnology (2017) 69:359–369, CC BY 4.0.