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    P1 Primary Cell 4D-Nucleofector<sup class=reg>®</sup> X Kit L
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    P1 Primary Cell 4D-Nucleofector® X Kit L
    Catalog #: V4XP-1012

    For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).

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    P1 Primary Cell 4D-Nucleofector<sup class=reg>®</sup> X Kit L
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    P1 Primary Cell 4D-Nucleofector® X Kit L
    Catalog #: V4XP-1024

    For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).

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    FlashGel<sup class=reg>®</sup> FLX RNA Prestain Loading Buffer (2X)
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    FlashGel® FLX RNA Prestain Loading Buffer (2X)
    Catalog #: 00316993
    FlashGel® FLX RNA Prestain Loading Buffer (2X loading buffer containing RNA stain), 1 mL – for use with FlashGel® FLX Cassettes
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    P4 Primary Cell 4D-Nucleofector<sup class=reg>®</sup> X Kit L
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    P4 Primary Cell 4D-Nucleofector® X Kit L
    Catalog #: V4XP-4012

    For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (100 µL format).

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    β-G-Blocker
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    β-G-Blocker
    Catalog #: N190

    β-G-Blocker, 5 × 5 mL

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    HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor
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    HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor
    Catalog #: CC-2505
    HMVEC-d Neo, Dermal Microvascular Endothelial, Neonatal, Single donor
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    Latitude<sup class=reg>®</sup> HT Precast Gel TBE
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    Latitude® HT Precast Gel TBE
    Catalog #: 57246

    Latitude® HT (24 × 14 cm) 2%, 4 × 25-well, 5 gels

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    FlashGel<sup class=reg>®</sup> FLX Cassettes
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    FlashGel® FLX Cassettes
    Catalog #: 00317260

    FlashGel® FLX Cassettes, 2.2% 12+1 single tier, 9 pack

    Ideal for testing 12 samples with small DNA fragments (e.g., PCR products).

    Separation range for DNA: 20 bp – 1 kb

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    FlashGel<sup class=reg>®</sup> FLX Cassettes
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    FlashGel® FLX Cassettes
    Catalog #: 00317259
    FlashGel® FLX Cassettes, 1.2% 16+1 double tier, 9 pack
     
    Easily screen 32 samples
     
    DNA: 100 bp – 10 kb separation range (separation of fragments >4kb will be improved by running longer at a lower voltage) 
     
    RNA: 0.5 – 9 kb separation range
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    FlashGel<sup class=reg>®</sup> FLX Cassettes
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    FlashGel® FLX Cassettes
    Catalog #: 00317258
    FlashGel® FLX Cassettes, 1.2% 12+1 single tier, 9 pack DNA: 100 bp – 10 kb separation range (separation of fragments >4kb will be improved by running longer at a lower voltage) RNA: 0.5 – 9 kb separation range
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1-10 of 124 results
  • TechSheet - Umbilical Vein Endothelial Cell Systems
    Technical information sheet for CloneticsTM HUVEC cells
  • Instructions for Use - HUVEC Culturing and Angiogenesis Inhibition Assay using Sartorius Incucyte®
    Protocol for Angiogenesis assay using HUVEC cells
  • Tube Formation Assay with Primary HUVECs
    In this study, we have optimized this assay using Lonza's Primary HUVECs and EGM®-2 Media
  • Expanded HUVECs for High-throughput Screening
    HUVECs are a good primary endothelial cell type for many in vitro studies and also for several high-throughput screening applications. Consequently, Lonza offers, HUVEC-XL. These cells are expanded to passage-3 from pooled P1-HUVECs and packaged at 10 million cells per ampoule.
  • Tube Formation Assay with HUVEC
    Human Umbilical Vein Endothelial Cells seeded on BME and the formation of endothelial tubes were monitored live with the CytoSMART™ Lux 10X System, the previous version of the CytoSMART™ 2 System. Time-lapse video was recorded for 22 hours at a frame interval of 1 frame every 5 minutes.
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  • HUVEC-Fibroblast Co-Culture - Angiogenesis
    Time-Lapse Video of Human Umbilical Vein Endothelial Cell Angiogenic Tube Formation in Co-Culture with Dermal Fibroblasts. Primary Human Umbilical Vein Endothelial Cells (HUVECs) were co-cultured with primary Human Normal Dermal Fibroblast cells at a ratio of 1:3 HUVECs: fibroblasts and a total cell density of 40,000 cells/well in a 96-well plate. Co-cultures were maintained for 5 days with medium changes every 2-3 days in EBMTM-2 medium supplemented with a custom concentration of VEGF (313 pM) to optimize angiogenic tube formation in this setting. Cells were stained with CellTracker® Dye from ThermoFisher. Purple lines indicate angiogenic tube formation detected by the live cell imaging software. At each frame, the software quantifies angiogenesis by counting the number of network branch points (i.e., the number of branches in each network of tubes) per mm2 and the mean network length (i.e., the average of the total network length of tubes per mm2).
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  • HUVEC-Fibroblast Co-Culture – Angiogenesis Inhibition
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  • Automated Cell Signaling Assays Using Primary HUVECs
    Lonza's Primary HUVECs are an excellent cell type to measure the effects of pharmacologically-important compounds on vial signaling pathways, and they provide increased data relevancy compared to cell lines.
  • Lonza Extends Industry-Standard HUVEC Product Line
    Lonza, the market leader in primary cells, has expanded its human primary cell portfolio with an improved version of Human Umbilical Vein Endothelial Cells (HUVEC) cultured in the absence of additive vascular endothelial growth factor (VEGF).
  • Instructions - HUVEC-XL™ Pooled Cell System
    Instructions for culturing Clonetics™ HIVEC-XL™Pooled Cells