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Detection of CRISPR Off-Target Effect with DISCOVER-Seq

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Article overview

Using longer single guides to increase editing efficiency

The authors describe a novel approach that leads to higher editing efficiency in CRISPR experiments

CRISPR-based genetic screen in physiological relevant primary cells

Review of "CRISPR-based Genetic Screen in Physiological Relevant Primary Cells" from Judd Hultquist.

Video protocol for CRISPR/Cas9 genome editing

Different techniques for genome editing in diverse cells and species.

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References

Wienert B, Wyman SK, Yeh CD, Conklin BR, Corn JE. CRISPR off-target detection with DISCOVER-seq. Nat Protoc. 2020 May;15(5):1775-1799

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