Product Overview
The X-VIVOTM 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2.
X-VIVOTM 10, catalog number 04-380Q contains L-glutamine, gentamicin, and phenol red.
THIS PRODUCT IS NOT FOR USE IN GMP MANUFACTURING, NOR HUMAN OR ANIMAL IN VIVO USE, INCLUDING USE AS A DILUENT OR AS AN EXCIPIENT, OR FOR DIAGNOSTIC USE. This product is for research use only. If you require GMP grade media, contact Lonza for more details.
Request More InfoBenefits
- Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.
- Formulations only contain human derived components
- Highly cited in scientific literature
- Clinical proven formulations providing process safety and constancy
- High productivity resulting in a high viable cell yield
- Serum Free media result in a more defined process and result in limited immune response
- Efficient transgene expression Desirable T-cell phenotype
Applications
- Proliferation of peripheral blood lymphocytes
- Proliferation of tumor infiltrating lymphocytes
- Cryopreservation of human tissue
- Cultivation of human monocytes and macrophages
- Cultivation of stem cells
- Cultivation of dendritic cells