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X-VIVOTM 10 Serum-free Hematopoietic Cell Medium

X-VIVOTM 10 Serum-free Hematopoietic Cell Medium

Catalog #: 04-380Q

X-VIVOTM 10, Serum free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 1 L

 

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Product Overview

The X-VIVOTM 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2.

X-VIVOTM 10, catalog number 04-380Q contains L-glutamine, gentamicin, and phenol red. 

THIS PRODUCT IS NOT FOR USE IN GMP MANUFACTURING, NOR HUMAN OR ANIMAL IN VIVO USE, INCLUDING USE AS A DILUENT OR AS AN EXCIPIENT, OR FOR DIAGNOSTIC USE. This product is for research use only. If you require GMP grade media, contact Lonza for more details.

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Benefits

  • Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.
  • Formulations only contain human derived components
  • Highly cited in scientific literature
  • Clinical proven formulations providing process safety and constancy
  • High productivity resulting in a high viable cell yield
  • Serum Free media result in a more defined process and result in limited immune response
  • Efficient transgene expression Desirable T-cell phenotype

Applications

  • Proliferation of peripheral blood lymphocytes
  • Proliferation of tumor infiltrating lymphocytes
  • Cryopreservation of human tissue
  • Cultivation of human monocytes and macrophages
  • Cultivation of stem cells
  • Cultivation of dendritic cells

Storage and Content

  • 1L bottle
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SDS, CoA, and Instructions

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

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  • References - X-Vivo™

    Overview of relevant references
  • TechSheet - X-VIVO™ Media Systems

    Instructions for how to use the X-VIVO™ Media Series
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Educational Material

Brochures, White Papers etc.

  • CAR T cells in Cancer Therapy

    A poster developed by Nature Reviews in conjunction with Lonza to present CAR T cells in cancer therapy. This poster demonstrates the recognition of tumor peptides by T cells, CAR T cell manufacturing, the types of chimeric antigen receptors and a timeline of CAR development over more than 30 years.
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