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Empowering NK Cell Immunotherapy Research Using a Novel Genome Editing Protocol

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Article overview

CRISPR/Cas9 based genome engineering of NK cells using Nucleofector® Technology

How to handle the natural killers for genomic engineering purposes?

Learn from the authors about the potential overcome of some limitation of NK cell-based therapies and check for the detailed protocol of Cas9 RNP transfection. 

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Video protocol for CRISPR/Cas9 genome editing

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Reprogramming of human PBMC into iPSC as a common method for disease research

PBMC collected from patients were reprogrammed into iPSC by Nucleofection. Generated IPSCs still carry the mutation causing the disease.
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References

Rautela J, Surgenor E,  Huntington ND. Efficient genome editing of human natural killer cells by CRISPR RNP. bioRxiv 2018

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