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NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid

Catalog #: CC-2540

Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells with Retinoic Acid containing ≥500,000 cells

 

Interested in reducing lab time? Lonza offers proliferating primary cells in a variety of convenient flask and plate formats. Request more information or quote now.

Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research

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Product Overview

Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza's Normal Human Bronchial Epithelial Cells (NHBE) are available with retinoic acid. 

All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Select Applications with Lonza's Human Bronchial Epithelial Cells -

  • A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
  • Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
  • Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.

Purchase our Bronchial Epithelial Cell Culture Kit (catalog no. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog no. CC-2540) and BEGMTMTM BulletKitTM Growth Media (catalog no. CC-3170)

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Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Storage and Content

1 x Cryopreserved ampule NHBE w/ RA containing ≥500,000 cells

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Instructions - Airway Epithelial Cell Systems

    Instructions for Proper Use and Culture of Airway Epithelial Cells
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • TechSheet - Bronchial, Tracheal Epithelial Cell Systems

    Technical information sheet for Clonetics™ Bronchial and Tracheal cells
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Educational Material

Brochures, White Papers etc.

  • B-ALI Bronchial Air Liquid Interface Media Kit, a Guaranteed 3D in vitro Model for Respiratory Research

    Lonza Whitepaper
  • Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System

    Understanding the differences between normal and diseased lung cells in 2D vs 3D culture.
  • Primary Human Airway Cells and Media

    Airway Cell and Media Reference Guide
  • Gene Expression Differences in Airway Epithelial Cells from Asthma or COPD Donors

    Access to diseased primary cells from patients diagnosed with COPD or asthma provides a convenient, biologically relevant model to assess genetic and in some cases phenotypic changes from normal, healthy donor tissues. In this study, we grew human bronchial epithelial (HBE) cells from normal, COPD-, and asthma-diagnosed human donor lung tissues in an air-liquid interface (ALI) model to assess potential phenotypic changes in vitro as well as provide a differentiated layer of cells to harvest for gene expression studies using the StellARray™ Gene Expression System.
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Primary Cells for Airway Research – Infographic

    Infographic showing how Lonza primary cells can aide in your airway research
  • Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media

    Reference to Help Get Started on Primary Cell Culture for ADMETox Applications
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Data

Bronchial Epithelial Cells in RAFT™ 3D Cell Culture System

Bronchial Epithelial Cells Grown in BEGM™ BulletKit™ and Cultured in RAFT™ 3D Cell Culture System

Data

Normal Human Bronchial Epithelial Cell

Normal human bronchial epithelial cell (NHBE) culture showing excellent packed cuboidal morphology

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BEGM™ Medium Available

Primary Cell Growth Medium

Our BEGMTM Bronchial Epithelial Cell Growth Medium is fully stocked. We appreciate your business and are dedicated to supporting your research needs.

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