Chemically defined, non-animal origin (NAO), serum-free medium
Chemically defined, non-animal origin (NAO), serum-free medium
ProCHO® 5 Protein-free CHO Medium Powder kit with HEPES and 0.1% Pluronic® F-68, without L-Glutamine, phenol red, hypoxanthine or thymidine, 500 L (NAO)
ProCHO® 4 Protein-free CHO Medium with 0.1% Pluronic® F-68, without L-glutamine, phenol red, hypoxanthine or thymidine, 1 L.
ProCHO® 5 protein free CHO medium, with HEPES and 0.1% Pluronic® F-68, without L-Glutamine, phenol red, hypoxanthine or thymidine, 1 L (NAO)
ProCHO® 4 Protein-free CHO Medium with 0.1% Pluronic® F-68, without L-glutamine, phenol red, hypoxanthine or thymidine, 1 L
ProCHO® 4 Protein-free CHO Medium with 0.1% Pluronic® F-68 and phenol red, without L-glutamine, hypoxanthine or thymidine, 1 L
XTREME® CHO Feed Liquid - CD, Protein free, Liquid, 1L
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit