For medium-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 4D-Nucleofector® 96-well Unit
PowerFeed® A Chemically Defined, Protein-free Basal Powder, without ferric citrate, 50L
Culture system containing EBM®-2 Basal Medium (CC-3156) and EGM®-2 MV Microvascular Endothelial Cell Growth Medium SingleQuots® supplements (CC-4147) required for growth of Microvascular Endothelial Cells
EBM® Plus Endothelial Basal Medium
Basal media required for growth of endothelial cells, phenol red free, 500 mL (supplements and growth factors sold separately)
X-VIVO® 15, Serum-free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 1 L
PowerFeed® A, CD , Protein free, Basal Powder without ferric citrate, 10L
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (20 µL format).
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Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Spanish)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Italian)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Chinese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENT™ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish. -
Instructions - PYROGENT™ and PYROGENT™ Plus (Chinese)Detailed instructions for performing the PYROGENT™ and PYROGENT™ Plus Gel Clot LAL Assay