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Cell Line Optimization 96-well Nucleofector® Kit

Cell Line Optimization 96-well Nucleofector® Kit

Catalog #: V4SC-9096

For rapid determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.

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Product Overview

The Cell Line Optimization 96-well Nucleofector® Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.

With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well NucleocuvetteTM Plate with different programs, primary cell optimizations are easily performed within one experiment. Using one 96-well Nucleocuvette® Plate and our pmaxGFPTM Vector as positive control, our three Cell Line Nucleofector® Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls.

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.  

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Benefits

  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
  • Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System

Applications

  • Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit

Storage and Content

0.675 mL Nucleofector® Solution SE

0.675 mL Nucleofector® Solution SF

0.675 mL Nucleofector® Solution SG

3 x 0.15 mL Supplement 1

50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

1 x 96-well Nucleocuvette® Plate

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Educational Material

Videos

  • How it Works - 96-well Shuttle™ System (Video Tutorial)

    Workflow overview for using the 96-well Shuttle™ System
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