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Lung Fibroblast Cell Culture Kit

Lung Fibroblast Cell Culture Kit

Catalog #: CC-2512B

Complete cell culture kit containing a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Fibroblast Growth Media (catalog no. CC-3132) for culture of lung fibroblast cells.

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Product Overview

Purchase our Lung Fibroblast Cell Culture Kit and get a discounted price on cells and media.

The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Fibroblast Growth Media (catalog no. CC-3132).

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Storage and Content

1 x Cryopreserved ampule of NHLF (CC-2512) containing ≥ 500,000 cells 

1 x FBMTM Basal Medium (CC-3131), 500 mL

1 x FGMTM-2 SingleQuotsTM Supplement Pack (CC-4126) containing:

1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Bottle FBS, 10.00 mL

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Data

NHLF – Human Lung Fibroblasts

Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was Horse anti Mouse, DI 2488 VectorLabs, used at 1:200 dilution. Nuclei were stained with Hoechst at 1:10,000.

Data

NHLF – Human Lung Fibroblasts

Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was , Goat anti Chicken Alexa 594, A11042 Invitrogen: Used at 1:200, Horse anti Mouse, DI 2488 VectorLabs: Used at 1:200  Nuclei were stained with Hoechst.

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