SG Cell Line 4D-Nucleofector™ X Kit S
SG Cell Line 4D-Nucleofector™ X Kit S
Product Overview
The SG Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection® conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well ShuttleTM System and 384-well Nucleofector® System
Applications
- Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
- Medium throughput applications
- Optimization of transfection conditions, e.g. substrate titrations
Storage and Content
1 x 0.675 mL SG Cell Line Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Raji Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of Raji cells with the SG Cell Line 4D-Nucleofector™ X Kit -
Ramos Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of Ramos cells with the SG Cell Line 4D-Nucleofector™ X Kit -
THP-1 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of THP-1 cells with the SG Cell Line 4D-Nucleofector™ X Kit -
CHO-S [Suspension] Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of CHO-S cells (Invitrogen) with the SG Cell Line 4D-Nucleofector™ X Kit -
NIH/3T3 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of NIH/3T3 cells with the SG Cell Line 4D-Nucleofector™ X Kit -
BA/F3 Transfection Protocol - 4D-Nucleofector™ Device
Instructions of use for transfection of BA/F3 cells (DSMZ) with the SG Cell Line 4D-Nucleofector™ X Kit -
H4 Transfection Protocol - 4D-Nucleofector™ X-Unit
Instructions of use for transfection of H4 cells with the SG Cell Line 4D-Nucleofector™ X Kit