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Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 MillionCatalog #: 2W-200Cryopreserved ampule of Human Peripheral Blood CD4+ T Cells containing ≥10 million cells, negatively selectedSales unit: each
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LAL Reagent Grade Multi-well PlatesCatalog #: 25-340
96-well individually wrapped microplate specific for endotoxin testing.
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100 bp DNA Ladder W/TYll Loading BufferCatalog #: 50321DNA Ladder, 100 bp W/TYll Loading BufferSales unit: each
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SimplyLoad® Tandem DNA LadderCatalog #: 50333
SimplyLoad® Tandem DNA Ladder, 500 µL
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Kinetic-QCL® Bulk Kinetic Chromogenic LAL Assay, 2040 Test KitCatalog #: 50-650NVBulk Kinetic Chromogenic LAL Assay for endotoxin detection for 2040 tests. Kit contains endotoxin. Assay sensitivity: 0.005 - 50 EU/mLSales unit: each
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SeaKem® GTG® AgaroseCatalog #: 50075
SeaKem® GTG® Agarose, 1 kg, ensures reliable digestion and ligation of recovered DNA fragments.
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100 bp Extended Range DNA Ladder W/TYIl Loading BufferCatalog #: 50322100 bp Extended Range DNA Ladder W/TYIl Loading BufferSales unit: each
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Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 2 MillionCatalog #: 2M-101D
Bone Marrow CD34+ Cells, ≥2 million cells, cryopreserved, positively selected
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MetaPhor® AgaroseCatalog #: 50180
MetaPhor® Agarose, 125 g, highest resolution agarose, ideal for small PCR fragments.
Sales unit: each
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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