Product Overview
The Primary Cell Optimization 4D-Nucleofector® X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
With the unique capability of the 4D-Nucleofector® X Unit to address individual wells of a 16-well Nucleocuvette® Strip with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using five 16-well Nucleocuvette® Strips and our pmaxGFPTM Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette® Strip can be used for optional fine tuning or performing the first experiment with the selected conditions. Cell Group-Specific Optimization using
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups:
- Endothelial cells (mammalian)
- Epithelial cells (mammalian)
- Fibroblasts (mammalian)
- Glial cells (mammalian)
- Neurons (mammalian)
- Smooth muscle cells (mammalian)
- Stem cells (human)
Before doing a complete or cell group-specific optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
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Benefits
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined in the 16-well Nucleocuvette® Strips are transferable to the 100 μL single Nucleocuvette® in the 4D-Nucleofector® X Unit
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
Applications
- Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit
Storage and Content
0.675 mL Nucleofector® Solution P1
0.675 mL Nucleofector® Solution P2
0.675 mL Nucleofector® Solution P3
0.675 mL Nucleofector® Solution P4
0.675 mL Nucleofector® Solution P5
4 x 0.15 mL Supplement 1
1 x 0.15 mL Supplement 3
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
6 x 20 µL 16-well Nucleocuvette® Strips