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P4 Primary Cell 4D-Nucleofector™ X Kit S

P4 Primary Cell 4D-Nucleofector™ X Kit S

Catalog #: V4XP-4032
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-NucleofectorTM X Unit (20 µL format).
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Product Overview

The P4 Primary Cell 4D-NucleofectorTM X Kit S is one of our five 4D-NucleofectorTM Kits suited for transfecting primary cells in the 20 µL 16-well NucleocuvetteTM Strip format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

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Benefits

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System

Applications

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

Storage and Content

1 x 0.675 mL P4 Primary Cell Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Transfection of Human Induced Pluripotent Stem Cells using Nucleofector® Technology

    Technical Reference Guide providing tips for transfecting iPSCs using the Nucleofector® Technology
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Educational Material

Brochures, White Papers etc.

  • Transfection of Human Induced Pluripotent Stem Cells using Nucleofector® Technology

    Technical Reference Guide providing tips for transfecting iPSCs using the Nucleofector® Technology
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