Perform the following steps before you begin media or cell preparation:
- Prepare a sterile surface
- A sterile field consist of a Class II biological safety cabinet with a front access opening and filtered laminar airflow, or an equivalent device.
- Determine the amount of medium required
- Review the growth area of Common Plastic ware table (see table on the right) to determine the amount of medium to be used.
- Sterile instruments and vessels required
- Sterile disposable serological pipettes
- Micropipettes and sterile pipette tips
- Adjustable multichannel pipette or repeating pipette.
- Sterile reservoir for use with multichannel pipette
- Sterile 15mL centrifuge tubes
- Cell culture flasks, or multiwall, flat-bottom tissue culture plates
- Hemacytometer or cell counter
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Other required supplies
- 70% alcohol (ethanol or isopropanol)
- Growth medium (cell type specific)
- Protective gloves and garments
- Trypan Blue
- Check the calibration on the humidified incubator. Incubator should be humidified and set to 5% CO2, 95% air and 37⁰C
- Check the calibration on the humidified incubator. Incubator should be humidified and set to 5% CO2, 95% air and 37⁰C.