Cell Line Optimization Nucleofector™ Kit for Nucleofector™ Device
Product Overview
The Cell Line Optimization NucleofectorTM Kit is the ideal tool to determine the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). It enables you to conveniently determine the optimal Nucleofection condition of your cell line of interest within one experiment. The kit contains two different Cell Line NucleofectorTM Solutions, V and L, each of which should be tested in combination with seven different NucleofectorTM Programs. It is suitable for 18 optimization reactions. Fine-tuning for optimal results can then be performed together with our Scientific Support Team.
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Benefits
- Efficient transfection of virtually any difficult-to-transfect cell line
- Simple and rapid optimization completed within just one experiment
Applications
- Optimization of Nucleofection conditions of virtually any difficult-to-transfect cell line with the NucleofectorTM II/2b Device
Content & Storage
Content
0.9 mL Cell Line NucleofectorTM Solution L
0.9 mL Cell Line NucleofectorTM Solution V
2 x 0.2 mL Supplement 1
2 x 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
20 Aluminum cuvette (100 µL)
20 Single use pipettes
