The medium-throughput module for the 4D-Nucleofector® Platform suited for transfection of hard-to-transfect cell lines or primary cells in 96-well format
Enables the direct Nucleofection® of cells in adherence in 24-well culture plates
The controlling unit of the 4D-Nucleofector® System is suited to handle various functional units for efficient transfection of hard-to-transfect cell lines and primary cells in different formats.
Accessory reservoir when using the second input tubing of an LV Nucleocuvette® Cartridge
Next generation large-scale Nucleofector® Platform for closed, scalable transfection of up to 1 billion cells – from research to GMP manufacturing
For use of the 4D-Nucleofector® 96-well Unit in a liquid handling system
Supports Nucleofection® of various cell numbers in 100 µL cuvettes or 20 µL 16-well strips
Flow-through reaction vessel for the 4D-Nucleofector® LV Unit PRO for research and process development. Easily scalable to up to 1 billion cells. Use in combination with P3 Nucleofector® Solution LV Sets and 4D-Nucleofector® LV Reservoirs.
For rapid determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
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Terms and ConditionsCD34+ cells BOGO
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf