PNGM™ Primary Neuron Growth Medium SingleQuots™ Supplements and Growth Factors
PNGM™ Primary Neuron Growth Medium SingleQuots™ Supplements and Growth Factors
PNGMTM Primary Neuron Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4462) contains all growth factors and supplements required for successful culture of our rat and mouse neurons.
Please note: PNGMTM SingleQuotsTM Kit (CC-4462) contains growth supplements only and does not include additional required PNBMTMBasal Media (CC-3652).
Product Overview
PNGMTM Primary Neuron Growth Medium SingleQuotsTM Supplements and Growth Factors were specifically developed for culturing embryonic primary rat and mouse neurons. For successful culture of neurons, growth factors and supplements should be combined with PNBMTM Basal Medium (CC-3256) (sold seperately).
PNBMTM Basal medium (CC-3256) and Singlequot Growth Factors (CC-4462) can be purchased seperately or in convenient PNGMTM BulletKitTM format (CC-4461)
Request More InfoBenefits
- Serum-free
- Allows for long-term maintenance and growth of neuronal cells
- Promotes development of high quality neuronal networks without the need of an astrocyte feeder layer
Storage and Content
- 1 x NSF-1, 4.0 mL
- 1 x L-Glutamine, 2.0 mL
- 1 x GA, 0.2 mL
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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ViaLightPlus Assay in PNGM™ Media of Rat Cortical Neurons
Comparison of survival of Clonetics™ Primary Rat Cortical Neurons cultured in PNGM™ Medium vs. Company A’s medium. Cryopreserved cells were thawed and plated in laminin-coated, 96 well plates at ~1000 cells per square mm (40% of the recommended seeding density) using either PNGM™ Medium or Company A’s medium. Cells were cultured without feeding for six days, at which time cell viability was assessed using the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay Kit. Results represent the average of 8 experiments.