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CompareCell Line Nucleofector® Kit VCatalog #: VCA-1003
For efficient transfection of cell lines, e.g. HepG2, HL-60, Jurkat, K-562 , MCF7, SH-SY5Y, or THP-1, in the NucleofectorTM I/II/2b System
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CompareSF Cell Line 96-well Nucleofector® KitCatalog #: V4SC-2096
For medium-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 4D-Nucleofector® 96-well Unit
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CompareSF Cell Line 384-well Nucleofector® KitCatalog #: V5SC-2010
For high-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 384-well HT Nucleofector® System.
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CompareSF Cell Line 384-well Nucleofector® KitCatalog #: V5SC-2002
For high-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 384-well HT Nucleofector® System.
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CompareSF Cell Line 96-well Nucleofector® Kit (960 RCT)Catalog #: V4SC-2960
For medium-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 4D-Nucleofector® 96-well Unit
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CompareHuman recombinant Insulin for Cell Culture, 5mg/ml, 1 LCatalog #: BE02-033Q
Human recombinant insulin for cell culture, 5mg/ml, 1 L bottle, sold separately or as part of CHO powder kits
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ComparePowerFeed® A Chemically Defined and Protein-free liquid Medium, 1 LCatalog #: BE02-044Q
PowerFeed® A Chemically Defined and Protein-free Liquid Medium, 1L
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CompareXtremeFeed® - Chemically Defined and Protein-free Basal Powder 200 LCatalog #: VPW-098Q
XtremeFeed®, chemically defined, protein free, Basal Powder 200L
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ComparePowerFeed® A – Chemically Defined and Protein-free Basal Powder 50 LCatalog #: VPW-095F
PowerFeed® A Chemically Defined, Protein-free Basal Powder, without ferric citrate, 50L
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ComparePowerFeed® A – Chemically Defined and Protein-free basal Powder 10 LCatalog #: VPW-095D
PowerFeed® A, CD , Protein free, Basal Powder without ferric citrate, 10L
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit