For efficient transfection of cell lines, e.g. HepG2, HL-60, Jurkat, K-562 , MCF7, SH-SY5Y, or THP-1, in the NucleofectorTM I/II/2b System
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RCGMTM SingleQuots® Kit (basal medium not included)
For efficient transfection of primary human DCs (dendritic cells) in the Nucleofector® I/II/2b Device
For efficient transfection of cell lines, e.g. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the Nucleofector® I/II/2b Device
For efficient transfection of parasites, e.g. Plasmodium berghei or Trypanosoma brucei, in Nucleofector® I/II/2b Device
For efficient transfection of primary mouse macrophages in the Nucleofector® I/II/2b Device
For efficient transfection of primary HCAECs in the Nucleofector® 2b Device
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Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Spanish)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Italian)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (German)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Chinese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENT™ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish.