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SG Cell Line 4D-Nucleofector® X Kit S

SG Cell Line 4D-Nucleofector® X Kit S

Catalog #: V4XC-3032

For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (20 µL format).

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Product Overview

The SG Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection® conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

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Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System

Applications

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions, e.g. substrate titrations

Storage and Content

1 x 0.675 mL SG Cell Line Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)

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SDS, CoA, and Instructions

Safety Data Sheets (SDS)

Choose a language to view the SDS.

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • 4D-Nucleofector® Protocol for Ramos

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Ramos
  • 4D-Nucleofector® Protocol for THP-1

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for THP-1
  • 4D-Nucleofector® Protocol for H4

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for H4
  • 4D-Nucleofector® Protocol for Colo-205

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Colo-205
  • 4D-Nucleofector® Protocol for BAF3

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for BAF3
  • 4D-Nucleofector® Protocol for Raji

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Raji
  • 4D-Nucleofector® Protocol for WEHI-231

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for WEHI-231
  • 4D-Nucleofector® Protocol for TD1 ORL UVA

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TD1 ORL UVA
  • 4D-Nucleofector® Protocol for NIH3T3

    Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NIH3T3
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Educational Material

Videos

  • How it Works - 4D-Nucleofector® System (Video Tutorial)

    Workflow overview for using the 4D-Nucleofector™ System
  • How it Works - 4D-Nucleofector™ X Unit (Video Tutorial)

    Workflow overview for using the 4D-Nucleofector™ X Unit
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Knowledge Center

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Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.