4D-Nucleofector® Core Unit
4D-Nucleofector® Core Unit
The controlling unit of the 4D-Nucleofector® System is suited to handle various functional units for efficient transfection of hard-to-transfect cell lines and primary cells in different formats.
Product Overview
The 4D-Nucleofector® Core Unit is the controlling unit of the 4D-Nucleofector® System. The different functional units can be added in a modular way to the 4D-Nucleofector® Core Unit and each is suited for different applications:
- X Unit: Supporting Nucleofection® of various cell numbers in different formats (16-well Nucleocuvette® Strips or 100 µL Nucleocuvette® Vessels
- Y Unit: Enabling adherent Nucleofection® in 24-well culture plates
- LV Unit: Large-scale transfection of up to 1x109 cells
- 96-well Unit: Higher throughput Nucleofection® Experiments in 96-well Nucleocuvette® Plates
Transfections per minute:
- X Unit (16-well Nucleocuvette® Strips): 16-32 reactions
- X Unit (100 µL Nucleocuvette® Vessels): 20-26 reactions
- Y Unit (Dipping Electrodes): 8-10 reactions
- LV Unit (LV Nucleocuvette® Cartridges): 2-3 mL
- 96-well Unit (96-well Nucleocuvette® Plate): 32 - 44 reactions
There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection® Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Technical Specifications
Dimensions: (w x h x d): 24.5×14.7×28 cm / 9.7×5.8×11.0
Weight: 4.4 kg ( 9.7 lb)
Power: 100 - 240 VAC
Frequency: 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environment non condensing
Voltage limitation: 1500 V
Current limitation: 100 A
Compatibility
The Core Unit can be used to operate the following functional units:
- X Unit
- Y Unit
- LV Unit
- 96-well Unit
This Core Unit is not compatible with the 96-well ShuttleTM Add-on
Benefits
- Controls attached functional units
- 8’’ touch screen to operate the system
- Intuitive operation software for seamless designing and saving individual experimental setups
- Includes USB and network connectivity for software update and data transfer
Applications
Controlling the 4D-Nucleofector® System
Storage and Content
1 UnitSDS, CoA, and Instructions
Safety Data Sheets (SDS)
SDS not applicable
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment -
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1 -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
Nucleofector® Technology Brochure
Stretching Transfection Dimensions
Educational Material
Brochures, White Papers etc.
Videos
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Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment -
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1 -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
Citation List
A list of selected and reviewed publications for the Nucleofector Technology with focus on 4D-Nucleofector System.
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4D-Nucleofector® Platform - The Story Of
See how breakthrough Nucleofector® Technology innovations in transfection and electroporation culminate in the next generation 4D-Nucleofector® System. -
How it Works - 4D-Nucleofector® System (Video Tutorial)
Workflow overview for using the 4D-Nucleofector™ System
