Primary Cell Optimization 384-well Nucleofector™ Kit
Primary Cell Optimization 384-well Nucleofector™ Kit
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Product Overview
The Primary Cell Optimization 384-well NucleofectorTM Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System.
Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control. Our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups:
- Endothelial cells (mammalian)
- Epithelial cells (mammalian)
- Fibroblasts (mammalian)
- Glial cells (mammalian)
- Neurons (mammalian)
- Smooth muscle cells (mammalian)
- Stem cells (human)
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Request More InfoBenefits
- High transfection efficiencies for primary cells
- Automated high-throughput electroporation
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined on the 384-well HT NucleofectorTM System are transferable to the 4D-NucleofectorTM System or the 96-well ShuttleTM Add-On
Applications
- Transfection of low cell numbers in the range of 2 x 104 to 1 x 106 cells
- Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System
Storage and Content
2.25 mL NucleofectorTM Solution P1
2.25 mL NucleofectorTM Solution P2
2.25 mL NucleofectorTM Solution P3
2.25 mL NucleofectorTM Solution P4
2.25 mL NucleofectorTM Solution P5
4 x 0.15 mL Supplement 1
1 x 0.15 mL Supplement 3
150 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 384-well NucleocuvetteTM Plate
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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