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SF Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-2024
For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-Nucleofector® X Unit (100 µL format).
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Human Recombinant Insulin for Cell Culture, 5mg/ml, 20ml bottle
Catalog #: BE02-033E20
Human Recombinant Insulin for Cell Culture, 5mg/ml, 20ml bottle, sold separately or as part of CHO powder kits
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Human Recombinant Insulin for Cell Culture, 5mg/ml, 100ml
Catalog #: BE02-033E
Human Recombinant Insulin for Cell Culture, 5mg/ml, 100ml bottle, sold separately or as part of CHO powder kits.
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eLearning: Working with Gel Clot
Catalog #: LAL-EL-3
This module describes how to work with the gel clot assay, including calculation of the Maximum Valid Dilution (MVD), product validation and the Initial Qualification (IQ) assay.
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Human Peripheral Blood CD8+ T Cells, Cryopreserved, 10 million cells
Catalog #: 2W-300
Cryopreserved ampule of Human Peripheral Blood CD8+ T Cells containing 10 million cells
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SE Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-1012
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (100 µL format).
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SE Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-1024
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (100 µL format).
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eLearning: Troubleshooting the BET
Catalog #: LAL-EL-6
This module explains different types of endotoxin test failures for gel clot and kinetic LAL assays, guides you through possible causes and suggests strategies to help avoid these issues.
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eLearning: Understanding the BET
Catalog #: LAL-EL-2
This introductory module introduces assay mechanisms, the basic assay requirements, the need for endotoxin controls and how Limulus Amebocyte Lysate (LAL) is made.
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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