SE Cell Line 4D-Nucleofector® X Kit S
SE Cell Line 4D-Nucleofector® X Kit S
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (20 µL format).
Product Overview
The SE Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 3T3-L1, diff., 3T3-L1, undiff.
- A-10, A7r5
- BHK-21, BJ
- C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
- DU145
- GH3
- HCT116, HeLa, HeLa S3
- IMR-90
- Jurkat (DSMZ), Jurkat E6.1
- KG-1a, L6
- MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
- NCTC clone 929
- Panc-1
- T/G-HA VSMC, T-47D, T84
- U2OS, U-87 MG
- WI-38
The respective cell type-specific Optimized Protocols are available available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Conditions are transferable between 4D Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
Applications
- Transfection of low cell numbers in the range of 5 × 104 to 1 × 106 cells
- Medium throughput applications
- Optimization of transfection conditions e.g. substrate titrations
Storage and Content
1 x 0.675 mL SE Cell Line Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μL)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
-
4D-Nucleofector® Protocol for MRC-5
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MRC-5 -
4D-Nucleofector® Protocol for IMR90
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for IMR90 -
4D-Nucleofector® Protocol for Jurkatclone E6.1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Jurkatclone E6.1 -
4D-Nucleofector® Protocol for T-47D
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for T-47D -
4D-Nucleofector® Protocol for Calu-3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Calu-3 -
4D-Nucleofector® Protocol for A7r5
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A7r5 -
4D-Nucleofector® Protocol for MDA-MB-468
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDA-MB-468 -
4D-Nucleofector® Protocol for HeLa cells
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HeLa cells -
4D-Nucleofector® Protocol for HCT116
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HCT116 -
4D-Nucleofector® Protocol for BJ
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for BJ
