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New cellular models to study Parkinson disease

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Article overview

Using longer single guides to increase editing efficiency

The authors describe a novel approach that leads to higher editing efficiency in CRISPR experiments

Video protocol for CRISPR/Cas9 genome editing

Different techniques for genome editing in diverse cells and species.

CRISPR/Cas9 based genome engineering of NK cells using Nucleofector® Technology

How to handle the natural killers for genomic engineering purposes?

Learn from the authors about the potential overcome of some limitation of NK cell-based therapies and check for the detailed protocol of Cas9 RNP transfection. 

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References

Beylina A, Langston RG, Rosen D, Reed X, Cookson MR. Generation of fourteen isogenic cell lines for Parkinson's disease-associated leucine-rich repeat kinase (LRRK2) Stem Cell Res. 2021 May; 53:102354