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P3 Primary Cell 4D-Nucleofector® LV Kit XL (5 x 1 RCT)
Catalog #: V4LP-3520
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
P2 Primary Cell 96-well Nucleofector® Kit
Catalog #: V4SP-2096
For medium-throughput transfection of primary cells, e.g. mouse macrophages or NHDF, when working with the 4D-Nucleofector® 96-well Unit.
P5 Primary Cell 96-well Nucleofector® Kit (960 RCT)
Catalog #: V4SP-5960
For medium-throughput transfection of primary cells, e.g. HUVEC, when working with the 4D-Nucleofector® 96-well Unit.
P5 Primary Cell 96-well Nucleofector® Kit
Catalog #: V4SP-5096
For medium-throughput transfection of primary cells, e.g. HUVEC, when working with the 4D-Nucleofector® 96-well Unit.
SG Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-3012
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (100 µL format).
SG Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-3024
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (100 µL format).
SG Cell Line 96-well Nucleofector® Kit (960 RCT)
Catalog #: V4SC-3960
For medium-throughput transfection of cell lines, e.g. Ba/F3, Raji or THP-1, when working with the 4D-Nucleofector® 96-well Unit
SG Cell Line 96-well Nucleofector® Kit
Catalog #: V4SC-3096
For medium-throughput transfection of cell lines, e.g. Ba/F3, Raji or THP-1, when working with the 4D-Nucleofector® 96-well Unit
P4 Primary Cell 96-well Nucleofector® Kit (960 RCT)
Catalog #: V4SP-4960
For medium-throughput transfection of primary cells, e.g. mouse B-cells or immature DCs, when working with the 4D-Nucleofector® 96-well Unit.
P4 Primary Cell 96-well Nucleofector® Kit
Catalog #: V4SP-4096
For medium-throughput transfection of primary cells, e.g. mouse B-cells or immature DCs, when working with the 4D-Nucleofector® 96-well Unit.
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Instructions - QCL-1000™ Assay (English)Detailed instructions for performing the QCL-1000™ Endpoint Chromogenic LAL Assay
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PYROSTAR™ ES-F Series BrochureThe PYROSTAR™ ES-F series of reagents are endotoxin-specific and unreactive to β-D-Glucan
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QCL-1000™ Endpoint Chromogenic LAL Assay - Technical TipsThe Importance of Glass Tubes /The Problem with PlasticIncubation Method96-well Plates1 EU/ml Standard PreparationVortexing and Mixing Potential Endotoxin Contaminants
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Instructions – Water for Cell CultureData sheet WFI
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Troubleshooting Guide - Testing Oil Solutions with PYROGENT® Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE®, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT® Gel Clot LAL Assay.
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Troubleshooting Guide - Endpoint Chromogenic LAL Assay Procedure (English)This is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate.
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Instructions - MycoZap™ Mycoplasma Elimination ReagentMycoZap™ Mycoplasma Elimination Reagent Instructions
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Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Assay Procedure Quick GuideThis is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate. (English)
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Overcoming Assay Inhibition or Enhancement Technical TipsOne of the most time-consuming aspects of endotoxin testing using Limulus Amebocyte Lysate (LAL) is pretreating samples to overcome assay inhibition and enhancement. All assays, independent of methodology, are standardized using endotoxin in water. Lonza offers three solutions for overcoming LAL testing inhibitions
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White-Paper LAL-vs-rFCLonza discusses the transistion From LAL to rFC Assays
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