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TheraPEAK® 4D-Nucleofector® LV Reservoirs, weldable (5 pcs)
Catalog #: G4LR-1501W
For use in combination with TheraPEAK® Nucleocuvette® Cartridges, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.
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Inspection Equipment 4D-Nucleofector® Single Cuvette
Catalog #: AXP-1003
For testing the functionality of your 4D-Nucleofector® X Unit for use with 100 µL Nucleocuvette® Vessels
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Inspection Equipment 4D-Nucleofector® 16-well Strip
Catalog #: AXP-1004
For testing the functionality of your 4D-Nucleofector® X Unit for use with 20 µL Nucleocuvette® Strips
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SF Cell Line 4D-Nucleofector® LV Kit L
Catalog #: V4LC-2002
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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P3 Primary Cell 4D-Nucleofector® LV Kit L
Catalog #: V4LP-3002
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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Primary Cell Optimization 96-well Nucleofector® Kit
Catalog #: V4SP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.
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SF Cell Line 4D-Nucleofector® LV Kit XL
Catalog #: V4LC-2020
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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P3 Primary Cell 4D-Nucleofector® LV Kit XL
Catalog #: V4LP-3020
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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SF Cell Line 4D-Nucleofector® LV Kit XL (5 x 1 RCT)
Catalog #: V4LC-2520
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the LV Nucleocuvette® Cartridge format when working with the 4D-Nucleofector® LV Unit
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SG Cell Line 4D-Nucleofector® X Kit S
Catalog #: V4XC-3032
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (20 µL format).
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Instructions - QCL-1000™ Assay (English)Detailed instructions for performing the QCL-1000™ Endpoint Chromogenic LAL Assay
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PYROSTAR™ ES-F Series BrochureThe PYROSTAR™ ES-F series of reagents are endotoxin-specific and unreactive to β-D-Glucan
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QCL-1000™ Endpoint Chromogenic LAL Assay - Technical TipsThe Importance of Glass Tubes /The Problem with PlasticIncubation Method96-well Plates1 EU/ml Standard PreparationVortexing and Mixing Potential Endotoxin Contaminants
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Instructions – Water for Cell CultureData sheet WFI
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Troubleshooting Guide - Testing Oil Solutions with PYROGENT® Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE®, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT® Gel Clot LAL Assay.
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Troubleshooting Guide - Endpoint Chromogenic LAL Assay Procedure (English)This is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate.
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Instructions - MycoZap™ Mycoplasma Elimination ReagentMycoZap™ Mycoplasma Elimination Reagent Instructions
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Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Assay Procedure Quick GuideThis is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate. (English)
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Overcoming Assay Inhibition or Enhancement Technical TipsOne of the most time-consuming aspects of endotoxin testing using Limulus Amebocyte Lysate (LAL) is pretreating samples to overcome assay inhibition and enhancement. All assays, independent of methodology, are standardized using endotoxin in water. Lonza offers three solutions for overcoming LAL testing inhibitions
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White-Paper LAL-vs-rFCLonza discusses the transistion From LAL to rFC Assays
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