For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (100 µL format).
Fixed-volume reaction vessel for the 4D-Nucleofector® LV Unit PRO for research and process development. Use in combination with P3 Nucleofector® Solution LV Sets.
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (20 µL format).
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (20 µL format).
For use in combination with TheraPEAK® Nucleofector® Solution Sets, supporting the use of the 4D-Nucleofector® LV Unit in a GMP environment.
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (100 µL format).
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Instructions - QCL-1000™ Assay (English)Detailed instructions for performing the QCL-1000™ Endpoint Chromogenic LAL Assay
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PYROSTAR™ ES-F Series BrochureThe PYROSTAR™ ES-F series of reagents are endotoxin-specific and unreactive to β-D-Glucan
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QCL-1000™ Endpoint Chromogenic LAL Assay - Technical TipsThe Importance of Glass Tubes /The Problem with PlasticIncubation Method96-well Plates1 EU/ml Standard PreparationVortexing and Mixing Potential Endotoxin Contaminants
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Instructions – Water for Cell CultureData sheet WFI
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Troubleshooting Guide - Testing Oil Solutions with PYROGENT® Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE®, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT® Gel Clot LAL Assay.
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Troubleshooting Guide - Endpoint Chromogenic LAL Assay Procedure (English)This is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate.
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Instructions - MycoZap™ Mycoplasma Elimination ReagentMycoZap™ Mycoplasma Elimination Reagent Instructions
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Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Assay Procedure Quick GuideThis is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate. (English)
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Overcoming Assay Inhibition or Enhancement Technical TipsOne of the most time-consuming aspects of endotoxin testing using Limulus Amebocyte Lysate (LAL) is pretreating samples to overcome assay inhibition and enhancement. All assays, independent of methodology, are standardized using endotoxin in water. Lonza offers three solutions for overcoming LAL testing inhibitions
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White-Paper LAL-vs-rFCLonza discusses the transistion From LAL to rFC Assays