For efficient transfection of cell lines, e.g. HepG2, HL-60, Jurkat, K-562 , MCF7, SH-SY5Y, or THP-1, in the NucleofectorTM I/II/2b System
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Cryopreserved ampule of Human Mesenchymal Stem Cells (hMSCs) containing ≥750,000 cells
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells with cell population frequency information
PowerCHO® 2 CD, with HEPES, Pluronic® F-68, without L-glutamine, phenol red, hypoxanthine or thymidine, 10 L bag
Cryopreserved ampoule of Normal Human Knee Articular Chondrocytes (NHAC-kn) containing ≥ 750,000 cells
PowerCHO® 2 Basal Powder, 100 L, chemically defined
For high-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 384-well HT Nucleofector® System.
For medium-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 4D-Nucleofector® 96-well Unit
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Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Spanish)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Italian)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Chinese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENTâ„¢ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish. -
Instructions - PYROGENTâ„¢ and PYROGENTâ„¢ Plus (Chinese)Detailed instructions for performing the PYROGENTâ„¢ and PYROGENTâ„¢ Plus Gel Clot LAL Assay