The Nucleofector® Technology uses a specific combination of optimized electrical parameters and cell type-specific solutions which enables transfer of a molecule directly into the cells’ nucleus. Due to this independency from cell proliferation, it allows for efficient transfection of even non-dividing primary cells (like resting T cells or neurons) and also speeds up expression.
Three specialized components are key to the successful and efficient transfection of primary cells or cell lines using a variety of substrates:
Since launching the Nucleofector® Technology by legacy Amaxa, we have introduced several innovations around those three components to expand its application to further cell types, lower and higher cell numbers or different throughputs.