Background Information
Current in vitro lung models often involve the use of Transwells® cell culture inserts for static cultures which lacks the biomechanical cues experienced in vivo that affects how cells behave.
More advanced lung models include microfluidic devices which provide miniaturisation and selected advantages. However, moving cells from a macroscopic culture environment of dishes, flasks and well-plates to microfluidic cell culture provides a potentially problematic obstacle requiring extensive revision of your existing culture protocols.
The Quasi Vivo® 600 is a milli-fluidic bioreactor designed to be fully compatible with standard 24 well plate Transwells® cell culture inserts, letting you easily transfer your standard static cell culture protocols into fluid flow and allowing cells to be cultured at an air liquid interface.
Chandorkar et al. (2017)1 recently demonstrated that the QV600 System provides significant benefits in the development of airway cells. The perfusion of media provides cells with a constant supply of nutrients and removal of waste, resulting in NHBE cells showing:
- Significantly accelerated and higher ciliogenesis
- Increased cilia movement
- Enhanced mucus production
- Improved barrier function
This improved functionality under perfusion can reduce the time taken for experimental procedures by up to two thirds.
Watch the webinar above to learn learn more.
References
1 Chandokar, P. et al. Fast-track development of an in vitro 3D lung/immune cell model to study Aspergillus infections. Scientific Reports. volume 7, Article number: 11644 (2017)
