One way to obtain a more standardized cell culture is to use cells for any downstream assay at consistent confluency. However, the determination of cell confluency is very user dependent. Often different users will judge the same cell culture to be have varying confluency, which poses large variation into any downstream assay. When well-versed cell biologists in our labs were asked to determine the confluency of a cell culture, we obtained results ranging from 50-70%. The CytoSMARTTM System overcomes this issue in offering a user-independent analysis of the cell confluency which is recorded in the CytoSMARTTM Project Page (here referred to as “cell coverage”). See also the images taken with the CytoSMARTTM Lux 10X System after 40% and 70% confluency in the image gallery.