Despite the many benefits of stem cell culture, there are some challenges involved in its clinical application. For example, it can be difficult to find suitable cell culture conditions to promote proliferation but also maintain the desired stem cell properties. Moreover, the need for a high quality and quantity of cells requires large-scale expansion of stem cells, followed by efficient and homogeneous differentiation into functional derivatives. Traditional methods using two-dimensional (2D) culturing techniques (for example, using plastic culture plates and xenogenic media) provide limited expansion and lose clonal and differentiation capacity after long-term passaging. Efficient and large-scale transfection of stem cells and generation of iPSCs has also proved challenging.
A range of innovative solutions are overcoming these challenges. For example, we offer a range of specialized xeno-free growth and differentiation media for stem cell cultures that have been designed to achieve optimum cell differentiation and performance. In addition, our RAFTTM 3D Cell Culture System uses a three-dimensional (3D) collagen scaffold to generate multicellular models that more closely mimic the in vivo environment.
Our advanced electroporation method, NucleofectorTM Technology, also offers a transfection solution for hard-to-transfect stem cells. This technology enables highly efficient non-viral transfection of primary adult stem cells, genome editing in iPSCs or embryonic stem cells, and episomal reprogramming of a variety of cells into iPSCs. It also enables flexible scaling to allow you to transfect the sufficient number and type of cells for your application.