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1,051 Documents found
  • Primary Cell Types Provide a Ready-to-Use Platform for Cell-Based Assays
    Poster presented at Miptec, Basel (CH) - Sep 21-24, 2010
  • Webinar - Presentation: Enhance Your Cell Culture with RAFT™ 3D Cell Culture System
    Lonza_Webinars_Webinar_-_Presentation_Enhance_Your_Cell_Culture_with_RAFT_3D_Cell_Culture_System.pdf
  • Immunofluorescence Visualization of Fibroblast or Cancer Cells in RAFT™ 3D Cell Cultures
    In this technical note, we show that immunofluorescence can be performed easily and routinely on RAFT™ 3D Cell Cultures. Primary human dermal fibroblasts (HDF) or the breast cancer cell line MCF7 were cultured in RAFT™ 3D culture system for up to 23 days prior to fixation and staining.
  • Determination of the Desired Cell Seeding Density in RAFT™ 3D Cell Cultures
    In this technical note, we give a few hints and tips to generate a series of RAFT™ 3D Culture densities easily into the same culture plate.
  • Creating RAFT™ 3D Cell Cultures with Different Thicknesses and Different Cell Types
    In this Technical Note, we show how RAFT™ 3D Cell Cultures with different thicknesses ranging from approximately 30 μm to 140 μm can be generated with only a few minor modifications to the standard protocol. RAFT™ Cultures with decreased thickness could be of interest when limited amounts of cells are available and there is a need to reduce number of cells used per RAFT™ Culture.
  • CRISPR – Tips and Tricks for Efficient Delivery into Primary Cells and Cell Lines
  • Optimization Protocol for Primary Cells - 96-well Shuttle™ Device
    Instructions of use for optimizing transfection conditions of primary cells with the Primary Cell Optimization 96-well Nucleofector™ Kit
  • Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System
    Understanding the differences between normal and diseased lung cells in 2D vs 3D culture.
  • Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System
    Mono- and co-cultures of Lonza’s normal and asthmatic bronchial epithelial cells and smooth muscle cells.
  • Transfection Protocol for Human T Cells (Unstim.) - 4D-Nucleofector™ LV Unit
    Instructions of use for transfection of unstimulated peripheral blood human T cells with the P3 Primary Cell 4D-Nucleofector™ LV Kit
  • Transfection Protocol for Human NK Cells - Nucleofector™ I/II/2b Device Series
    Instructions of use for transfection of human natural killer cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
  • The Importance of Cell-subtype Selection in Creation of a 3D Liver Fibrosis Model
    Data sheet detailing 3D liver fibrosis model in collaboration with Visikol
  • Webinar - Presentation: Enhance Your Cell Culture with RAFT™ 3D Cell Culture System (pptx)
    Lonza_Webinars_Webinar_-_Presentation_Enhance_Your_Cell_Culture_with_RAFT_3D_Cell_Culture_System.pptx
  • Transfection Protocol for Mouse B Cells - 96-well Shuttle™ Device
    Instructions of use for transfection of stimulated mouse B cells with the P4 Primary Cell 96-well Nucleofector™ Kit
  • Basic Transfection Protocol for Endothelial Cells - 96-well Shuttle™ Device
    Instructions of use for transfection of primary mammalian endothelial cells with the P5 Primary Cell 96-well Nucleofector™ Kit
  • Transfection Protocol for Human B Cells - 96-well Shuttle™ Device
    Instructions of use for transfection of human B cells with the P3 Primary Cell 96-well Nucleofector™ Kit
  • Basic Transfection Protocol for Epithelial Cells - 96-well Shuttle™ Device
    Instructions of use for transfection of primary mammalian epithelial cells with the P1 or P3 Primary Cell 96-well Nucleofector™ Kit
  • Basic Transfection Protocol for Neural Cells - 96-well Shuttle™ Device
    Instructions of use for transfection of primary mammalian neurons with the P3 Primary Cell 96-well Nucleofector™ Kit
  • TechSheet - Rat Cardiac Myocyte Cell System
    TechSheet - Rat Cardiac Myocyte Cell System
  • Instructions - Rat Cerebellar Neuronal Cell System
    Instructions for proper use and culture of Rat Brain Cerebellar Neurons