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Primary Cell Optimization 4D-Nucleofector™ X Kit
Catalog #: V4XP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-NucleofectorTM X Unit.
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma for B-ALI™ Culture
Catalog #: 00194911S
DHBE-As, Bronchial Epithelial Cells from donors having Asthma for B-ALI™ (Bronchial Air Liquid Interface) cell culture
P4 Primary Cell 4D-Nucleofector™ X Kit L
Catalog #: V4XP-4024
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-NucleofectorTM X Unit (100 µL format).
Human Bone Marrow, CD34+ Depleted, MNC, 100M
Catalog #: 4M-105
all CD34 isolation are done by using Indirect Isolation kits
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor
Catalog #: 00192907
Cryopreserved ampule of Neonatal Normal Human Epidermal Keratinocytes (NHEK-Neo), Single Donor, containing ≥ 500,000 cells
P4 Primary Cell 4D-Nucleofector™ X Kit S
Catalog #: V4XP-4032
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-NucleofectorTM X Unit (20 µL format).
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B-ALI Immune-Airway Co-Culture Model: Normal Human Bronchial Epithelial cells and Peripheral Blood Mononuclear Cells - Instructions for useProtocol to culture B-ALI NHBE cells with PBMCs
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Transfection Protocol for Human B Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human B cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Mouse B Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of stimulated mouse B cells with the P4 Primary Cell 96-well Nucleofector™ Kit
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TechSheet – Amphotericin BHow to use Amphotericin B (17-836)
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Transfection Protocol for Human B Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human B cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Transfection Protocol for Mouse B Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of stimulated mouse B cells with the P4 Primary Cell 4D-Nucleofector™ X Kit
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Transfection Protocol for Human B Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human B cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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Transfection Protocol for Mouse B Cells (stim.) - Nucleofector™ I/II/2b DeviceInstructions of use for transfection of stimulated mouse B cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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TechSheet – Penicillin-Streptomycin-Amphotericin BHow to use Pen/Strep/Amph (17-745)
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SourceBook Appendix B - Agarose Physical Chemistry1. Agarose Physical Chemistry 2. References
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