Assay designed to measure toxicity in mammalian cells and cell lines in culture
Supplements and growth factors required for culture of Endothelial Cells (basal media sold separately)
Cryopreserved ampule of Neonatal Normal Human Epidermal Keratinocytes (NHEK-Neo), Single Donor, containing ≥ 500,000 cells
Cryopreserved Human Neonatal Epidermal Keratinocytes, Pooled, ≥ 500,000 cells
Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 MV Microvascular Endothelial Cell Growth Medium SingleQuotsTM supplements (CC-4147) required for growth of Microvascular Endothelial Cells
Cryopreserved Human Adult Epidermal Keratinocytes (NHEK-Ad) from a single donor, ≥ 500,000 cells
FlashGelTM DNA Cassettes, 1.2% 12+1 well single-tier, 9 pack
Separation: 50 bp - 4 kb (up to 10 kb with lower voltage/longer run)
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FlashGelTM DNA Cassettes, 2.2% 12+1 well single-tier, 9 pack
Ideal for testing 12 samples with small DNA fragments (e.g. PCR products)
Separation: 10 bp - 1 kb
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Instructions – Water for Cell CultureData sheet WFI
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Overcoming Assay Inhibition or Enhancement Technical TipsOne of the most time-consuming aspects of endotoxin testing using Limulus Amebocyte Lysate (LAL) is pretreating samples to overcome assay inhibition and enhancement. All assays, independent of methodology, are standardized using endotoxin in water. Lonza offers three solutions for overcoming LAL testing inhibitions
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Troubleshooting Guide - Testing Oil Solutions with PYROGENT™ Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE™, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT™ Gel Clot LAL Assay.
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White PaperSimple and easy monitoring of three different cancer-relevant assays – Tube formation assay, 2D cell migration assay and 3D cell invasion assay
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White PaperLive Cell Monitoring with the CytoSMART™ System: Application Examples
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