Test conditions shared by other users
In addition to data generated by Lonza, our comprehensive cell database also contains data on Nucleofection conditions that were shared by other users and which have provided sufficient results for their purposes. You may apply these conditions to your cell type of interest, but please be aware that a more comprehensive optimization using options A and B described above may provide better results for your specific cell clone or source.
Using pmaxGFPTM Positive Control for Transfection Optimization
Regardless of what type of substrate you ultimately want to transfect for your application (plasmid, siRNA, protein or chemical compound), we always recommend using our pmaxGFPTM Vector for optimization. Once Nucleofection conditions are determined with the pmaxGFPTM Vector, they can be used with your specific substrate (e.g. plasmid DNA, mRNA, siRNA, shRNA vector, peptide, ribonucleoprotein). Using the pmaxGFPTM Vector for optimization eliminates a number of variables, such as the nature and quality of preparation of your substrate and the timing of analysis.
Fine-Tuning of Transfection Results (optional)
If results of your optimization experiment are not satisfactory yet, there is always the option to further fine-tune results. For the 4D-NucleofectorTM X Unit, the 96-well ShuttleTM Add-On or the HT NucleofectorTM System, this can be achieved with the aid of our fine tuning matrix. For platforms that do not offer such a matrix (4D-NucleofectorTM Y Unit or NucleofectorTM 2b Device), or for additional advice, please contact our Scientific Support Team.