How can you choose the best transfection method for your cells? Well, the ideal approach should offer high transfection efficiency, low toxicity and yield reproducible results. From a practical perspective, it should also be as quick and straightforward as possible to carry out.
To help you select the optimal transfection tool, consider these key questions:
- Which cell type are you using – primary cells, stem cells, cell lines, adherent cells, suspension cells etc.?
- Do you need to achieve stable or transient transfection?
- What molecule will you transfect (DNA, RNA or protein)?
- What is the transgene packaging capacity (i.e. what is the size of your construct)?
- Are there any general safety concerns? If so, what are they?
- Have you considered all the practical issues (e.g. cost, time, facilities/expertise available etc.)?
To further help you select the best way to transfect your cells, we’ve created a handy table comparing all the available transfection methods, highlighting their relative advantages and disadvantages (see below). You can find more information on transfection techniques by downloading our free pdf resource: ‘An Introduction to Transfection Methods – Technical Reference Guide’.