Performing a full optimization using Optimization Protocols
For each NucleofectorTM Platform, we offer an optimization protocol for cell lines and for most platforms, we offer an optimization protocol for primary cells. For the NucleofectorTM I/II/2b Device, we do not have an optimization protocol for primary cells due to the large number of different cell type specific solutions. Instead, we offer cell group-specific optimization protocols – refer to option B below.
Using our pmaxGFPTM Vector as positive control, a set of NucleofectorTM Solutions is tested in combination with a pre-selected set of programs plus controls. Especially with our NucleofectorTM Platforms that offer small scale multi-well formats (e.g. 4D-NucleofectorTM X Unit or 96-well ShuttleTM Add-On), such optimizations are straightforward, saving time and material. Established conditions are transferable between different platforms and scales (384-well HT NucleofectorTM System ↔ 96-well ShuttleTM Add-On ↔ 4D-NucleofectorTM X Unit ↔ 4D-NucleofectorTM LV Unit).