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Pharma & Biotech

4D-NucleofectorTM Y Unit

Catalog #: AAF-1002Y
Enables the direct Nucleofection of cells in adherence in 24-well culture plates

Product Overview

Adherent Nucleofection Using the Y Unit - Electroporation-based methods have so far required cells to be in suspension for transfection. The NucleofectorTM Technology entered a new era and allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state. The Y Unit of the 4D-NucleofectorTM System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection.

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


Technical Specifications

Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0
Weight: 5.0 kg (11.0 lb)
Power: 100 - 240 VAC Frequency 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environnement non condensing Voltage limitation: 1500 V
Current limitation: 100 A


  • Pre- and post Nucleofection culture in 24-well culture plates
  • Nucleofection of cells at any time point during this culture period, i.e. at a later developmental stage
  • Transfection efficiencies up to 70% combined with high viabilities
  • Compatible with CloneticsTM Primary Animal Neurons


  • Enables Nucleofection of cells in adherence in 24-well culture plates


Content & Storage


1 Unit


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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On-site Demonstration of 4D-NucleofectorTM Technology

4D-NucleofectorTM X and  Y Unit

Are you interested in seeing the NucleofectorTM Technology for medium-throughput applications in your lab and how it performs for your cells? One of our transfection specialists can come to your lab, introduce you to your preferred platform and perform a transfection experiment with your cell type of interest.