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Pharma & Biotech

4D-NucleofectorTM Core Unit

Catalog #: AAF-1002B
The controlling unit of the 4D-NucleofectorTM System is suited to handle various fuctional units for efficient transfection of hard-to-transfect cell lines and primary cells in different formats.

Product Overview

The 4D-NucleofectorTM Core Unit is the controlling unit of the 4D-NucleofectorTM System. The different functional units can be added in a modular way to the 4D-NucleofectorTM Core Unit and each is suited for different applications:

  • X Unit: Supporting Nucleofection of various cell numbers in different formats (16-well NucleocuvetteTM Strips or 100 µL NucleocuvetteTM Vessels 
  • Y Unit: Enabling adherent Nucleofection in 24-well culture plates 
  • LV Unit: Large-scale transfection of up to 1x109 cells 
  • 96-well ShuttleTM Add-on: Higher throughput Nucleofection in 96-well NucleocuvetteTM Plates


Transfections per minute:

  • X Unit (16-well NucleocuvetteTM Strips): 16-32 reactions
  • X Unit (100 µL NucleocuvetteTM Vessels): 20-26 reactions
  • Y Unit (Dipping Electrodes): 8-10 reactions 
  • LV Unit (LV NucleocuvetteTM Cartridges): 2-3 mL

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


Technical Specifications

Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0
Weight: 4.4 kg ( 9.7 lb)
Power: 100 - 240 VAC
Frequency: 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environnement non condensing Voltage limitation: 1500 V
Current limitation: 100 A


  • Controls attached functional units
  • 5.7’’ foldable touch screen to operate the system
  • Intuitive operation software for designing and saving individual experimental setups
  • USB port for software update and data transfer
  • Includes USB and serial connectivity for the 96-well ShuttleTM Add-on


  • Controlling the 4D-NucleofectorTM System

Content & Storage


  • 1 Unit


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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On-site Demonstration of 4D-NucleofectorTM Technology

4D-NucleofectorTM X and  Y Unit

Are you interested in seeing the NucleofectorTM Technology for medium-throughput applications in your lab and how it performs for your cells? One of our transfection specialists can come to your lab, introduce you to your preferred platform and perform a transfection experiment with your cell type of interest.