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Pharma & Biotech

Cell Line Nucleofector™ Kit V

Catalog #: VACA-1003

DISCONTINUED - For efficient transfection of cell lines, e.g. HepG2, HL-60, Jurkat, K-562 , MCF7, SH-SY5Y, or THP-1, in the NucleofectorTM I/II/2b Device



This NucleofectorTM Kit size is no longer available. Please choose a different size from the menu below.


Product Overview

The Cell Line NucleofectorTM Kit V is one of our five NucleofectorTM Kits suited for transfecting cell lines in the NucleofectorTM 2b Device. This kit has been determined to be optimal for transfecting following cell types:

  • 32D
  • 3T3-L1 pre-ad
  • A20
  • A375
  • A7r5
  • AGS
  • ARPE-19
  • B16-F10
  • BA/F3 (DSMZ)
  • C2C12
  • C6
  • Capan-1
  • CHO [suspension]
  • CHO-S [suspension] (Life Technologies)
  • COS-1
  • HaCaT
  • HCT116
  • HEK293
  • HepG2
  • HL-60
  • HUV-EC-C
  • Jurkat
  • K-562 (DSMZ)
  • MCF7
  • MDA-MB-231
  • MDA-MB-468
  • NB-4 (DSMZ)
  • NCTC clone 929
  • Neuro-2a [N2a]
  • NG108-15
  • P19
  • PC-12
  • PC-3
  • Raji
  • Ramos
  • RAW 264.7
  • RBL-1
  • S49
  • Saos-2
  • Schneider‘s Drosophila Line 2
  • SH-SY5Y
  • SK-N-SH
  • SK-OV-3
  • SW480
  • T/C-28 a2
  • T/G
  • T-47D
  • THP-1
  • U-2 OS
  • Vero

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.



  • High transfection efficiencies in difficult-to-transfect cell lines


  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Content & Storage


2 x 0.45 mL NucleofectorTM Solution V
2 x 0.1 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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